nagene
products
Precision in every gene, innovation in every step – offering customer-focused, flexible solutions, all manufactured and quality tested in Austria.
Plasmid (pDNA)
The following table shows an estimate of our special express production times, based on non-complex sequences of up to 1500 base pairs.
| Scale | Culture volume | Yield | BSE/TSE & Endotoxin Free | TAT 5 days |
| Mini Prep | 1 mL - 5mL | 1 μg - 3 μg |  |
|
| Mini Prep | 50 mL - 100 mL | 15 μg - 50 μg | |
|
| Maxi Prep | 100 mL - 250 mL | 100 μg - 500 μg | |
|
| Mega Prep | 0.5 L - 2.5 L | 500 μg - 2.5 mg | |
|
| Giga Prep | 2.5 L | 2.5 mg - 10 mg | |
+1 day |
| High Scale | > 10 mg on request |
| Definition of Complexity | |
| GC rich sequences | starting from 75% |
| Hairpin structure | 25 bp |
| Direct repeats | 25 bp |
| Specification | |
| 100 bp - 10000 bp, larger on request | |
| Vectors: Customized | |
| Biosafety level 1 | |
| Concentration: Adjustment on request | |
| Linearization possible | |
Process from
Start to Finish
Capacity in 2024 = 12 genes/day (1500 bp)
Capacity in 2025 = 64 genes/day (1500 bp)
Quality Control Methods
| Method | Specification |
| Appearance | No visible particle, clear solution |
| Restriction digest | Expected digest pattern |
| Complete plasmid DNA sequencing (NGS) | Expected Sequence |
| DNA concentration, UV 260 | Target concentration ± 15% (1 μg/μl) |
| Purity, UV 260/280 | 1.6 – 2.0 |
| Host cell protein | < 10 μg/mg |
| Residual Host DNA | < 5% total DNA |
| Method | Specification |
| Homogenity | > 80% supercoiled |
| Endotoxin | ≤ 0.1 EU/μg |
| Bioburden | < 1 CFU/ml |
| Mycoplasma | Absence |
Product
Documentation
- Batchrecord
- Certificate of Conformance
- Quality Report
- Certificate of Analysis
State-of-the-art
equipment
Fully automated, walk-away synthesis
Plug-and-play integration
Flexible and very user-friendly
Parallel synthesis in 96-well plates
Flexibility for up to 96 oligos per run
Synthesize labeled and unlabeled oligos
15-120 nt de novo synthesis (A, T, G, and C)
Custom iDNA of 1-45 nt available
Default 5'-phosphate
15- to 30-min setup time per run
Same-day synthesis of 15-30 nt oligos enables two runs in 24 hours
Synthesize oligos overnight for next-day use
Ready-to-use oligos for molecular biology and genomics applications
Modify oligos with fluorophores, quenchers, and biotin
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High-precision measurements (Q values from Q30 to Q50)
Analyzes native DNA or RNA at any fragment length
Enables quick access to critical information and deeper
Integrated compute
Fast and flexible to use
The GridION has a compact size
Parallel execution of up to 5 nanopore experiments with fully integrated high-performance computing
Nanopores read the length of the DNA or RNA presented to them - from short to ultra-long (longest >4 MB)
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Fast and accurate measurements
Highly specific for DNA, RNA and proteins
Ease of use and specific quantification
Superiority over UV absorption methods in sensitivity and specificity
High sensitivity and accuracy
Distinguishes between different molecule types and states
Intuitive operation with instant results and easy data export
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